منابع مشابه
Modeling genome coverage in single-cell sequencing
MOTIVATION Single-cell DNA sequencing is necessary for examining genetic variation at the cellular level, which remains hidden in bulk sequencing experiments. But because they begin with such small amounts of starting material, the amount of information that is obtained from single-cell sequencing experiment is highly sensitive to the choice of protocol employed and variability in library prepa...
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We present a survey of single-cell whole-genome amplification (WGA) methods, including degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), multiple displacement amplification (MDA), and multiple annealing and looping-based amplification cycles (MALBAC). The key parameters to characterize the performance of these methods are defined, including genome coverage, uniformity, repr...
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Introduction: The emergence of single-cell RNA-sequencing (scRNA-seq) technology has provided new information about the structure of cells, and provided data with very high resolution of the expression of different genes for each cell at a single time. One of the main uses of scRNA-seq is data clustering based on expressed genes, which sometimes leads to the detection of rare cell populations. ...
متن کاملA Graph-Based Clustering Approach to Identify Cell Populations in Single-Cell RNA Sequencing Data
Introduction: The emergence of single-cell RNA-sequencing (scRNA-seq) technology has provided new information about the structure of cells, and provided data with very high resolution of the expression of different genes for each cell at a single time. One of the main uses of scRNA-seq is data clustering based on expressed genes, which sometimes leads to the detection of rare cell populations. ...
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A b s t r a c t 1 7 0 0 w o r d s m a x i m u m T a r d i g r a d e s a r e s m a l l (< 1 m m) a q u a t i c E c d y s o z o a n s t h a t o c c u p y a d i s t i n c t p h y l u m , T a r d i g r a d a. a n d d e m o n s t r a t e d t h a t a s m a n y a s 1 7 .
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ژورنال
عنوان ژورنال: Current Opinion in Biotechnology
سال: 2012
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2011.11.018